Ecalbio researchers firstly develop Patulin ELISA Test Kit in the world ,this kit is widely used in fruit industries, fruit companies, goverment quality control,etc.Patulin is a mycotoxin produced by a variety of molds, in particular, Aspergillus and Penicillium and Byssochlamys. Most commonly found in rotting apples, in general the amount of patulin in apple products is viewed as a measure of the quality of the apples used in production. In addition, patulin has been found in other foods such as grains, fruits, and vegetables. While not considered a particularly potent toxin, a number of studies have shown patulin to be genotoxic, which has led to some to theorize that it may be a carcinogen, although animal studies have remained inconclusive.
Patulin has shown antimicrobial properties against some microorganisms.Several countries have instituted patulin restrictions in apple products. The World Health Organization recommends a maximum concentration of 50 μg/L in apple juice. In the European Union, the limit is set to 50 micrograms per kilogram (μg/kg) in both apple juice and cider, and to half of that concentration, 25μg/kg, in solid apple products and 10 μg/kg in products for infants and young children. These limits came into force on 1 November 2003.
Principle
Patulin ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of the quantitative analysis of Patulin in Fruit and its products.
The coupling antigens are coated on the micro-well stripes. The Patulin in the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Patulin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration.
The optical density (OD) value of the sample has a negative correlation with the Patulin in it. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the Patulin concentration in the sample.
Detection time : 50 min
Sensitivity : 0.2ppb (μg/kg)
Background value for purely negative sample:<1 ppb
