β-lactam antibiotics (beta-lactam antibiotics) are a broad class of antibiotics, consisting of all antibiotic agents that contain a β-lactam ring in their molecular structures. This includes penicillin derivatives (penams), cephalosporins (cephems), monobactams, and carbapenems.Most β-lactam antibiotics work by inhibiting cell wall biosynthesis in the bacterial organism and are the most widely used group of antibiotics in animal .
Especially for mastitis, a bacterial infection of the dairy cow udder, they are the first line treatment. For the reasons of consumer protection and process safety in the dairy industry, in Commission Regulation (EU) No 37/2000 Maximum Residue Limits (MRLs) for 7 penicillins in food of animal origin were established.
Ecalbio Company researchers develop ELISA method for determination of β-lactam antibiotics in animal original food, which is high effective,s ensitivity, rapid comparing with LC-MS and HPLC.
The coupling antigens are pre-coated on the micro-well stripes. The β-Lactams in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-β-Lactams antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with β-Lactams concentration in the sample. This value is compared to the standard curve and the content of the corresponding β-Lactams is subsequently obtained.
Technical specifications
Sensitivity : 0.1 ppb Detect time: Approx. 75 min
Detection limit:
Tissue, liver, honey, shrimp, fish, milk ........... 2 ppb
Recovery rate:
Tissue, liver, honey, shrimp, fish, milk....... 85 ±10%
Cross-reaction rate:
Penicillin .....................100%
Ampicillin.....................90 %
Amoxicillin................... 80 %
Nafcillin ...................... 110%
Amoxicillin ...................80%
Piperacillin....................80%
Azlocillin...................... 90%
Cloxacillin.................... 75%
Dicloxacillin...................50%
Oxacillin ....................... 50%
Metampicillin..................50%
Carbenicillin...................10%
Ticarcillin...................... 10%
Cephalosporins..............<1%
96 tests/kit
