Tetrodotoxin, frequently abbreviated as TTX, is a potent neurotoxin. Its name derives from Tetraodontiformes, an order that includes pufferfish, porcupinefish, ocean sunfish or mola, and triggerfish, several species that carry the toxin. Although tetrodotoxin was discovered in these fish and found in several other animals (e.g., blue-ringed octopus, rough-skinned newt, and Naticidae) it is actually produced by certain symbiotic bacteria, such as Pseudoalteromonas tetraodonis, certain species of Pseudomonas and Vibrio, as well as some others that reside within these animals.
The Material Safety Data Sheet for TTX lists the oral median lethal dose (LD50) for mice as 334 μg per kg. Assuming the lethal dose for humans is similar, 25 milligrams (0.000881 oz) of tetrodotoxin would be expected to kill half of the group of 75 kg (165 lb) people that ingested it. The amount needed to reach a lethal dose by injection is much smaller, 8 μg per kg, or a little over one-half milligram (0.00002 oz) to kill a 75 kg (165 lb) person.
In 2012, Ecalbio team develop Tetrodotoxin (TTX) Rapid Test Kit, which can used to detect fish quickly. Recently, we develop Tetrodotoxin (TTX) ELISA kit,based on the competitive enzyme immunoassay for the detection of Tetrodotoxin in the tissue, liver, honey, intestine, shrimp, fish, milk and feed etc. The coupling antigens are pre-coated on the micro-well stripes. The Tetrodotoxin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-TTX antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Tetrodotoxin concentration in the sample. This value is compared to the standard curve and the content of the corresponding Tetrodotoxin is subsequently obtained.
This kit of Sensitivity is 8ppb, detect time is 75 min, 96 tests/kit.Result analysis as following:
The mean values of the absorbance values is obtained for the average OD value (B) of the sample and the standard solution divided by the OD value (B0) of the first standard solution (0 standard) and subsequently multiplied by 100%, that is,
B —the average OD value of the sample or the standard solution
B0—the average OD value of the 0 ng/mL standard solution
Percentage of absorbance value = B/B0* 100%
Draw the standard curve with the absorption percentages of the standard solution and the Tetrodotoxin semilogarithm values of the Tetrodotoxin standard solution (ng/mL) as Y- and X-axis, respectively. Read the corresponding concentration of the sample from the standard curve by incorporating its absorption percentage into the standard curve. The resulting value is subsequently multiplied by the corresponding dilution fold, finally obtaining the actual concentration of Tetrodotoxin in the sample.Using the professional analyzing software of this kit will be more convenient for the accurate and rapid analysis of a large amount of samples. (Please contact us for this software) .
B/B0 (%)
