Bacitracin ELISA Kit

Bacitracin ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of bacitracin in the pork, beef and tissue,etc.

Sensitivity : 2.0ppb

Detect time: 45min

Specification: 96tests/kit

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Description

1. Principle

Bacitracin ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of bacitracin in the pork, beef and tissue,etc.

The coupling antigens are coated on the micro-well stripes. The Bacitracin in the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Bacitracin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration.

The optical density (OD) value of the sample has a negative correlation with the Bacitracin in it. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the Bacitracin concentration in the sample.

2. Technical specifications

Sensitivity : 2.0ppb

Detect time: 45min

Detect Limit：

Tissue..........................................2 ppb

Cross-reaction rate:

Tissue..........................................100%

Recovery rate:

Tissue...........................................85 ± 20%

Sample preparation

-Tissue, Chicken, pork, porcine liver, shrimp or fish (Lower detect:30ppb)
1.Take 1±0.05 g of the homogenized sample into 10mL centrifuge tube, add 4 mL Redissolving solution, mix thoroughly for 2 min.
2.Centrifuge at above 4000 r/min at room temperature (20-25 ℃) for 5 min.
3.Take 50 µL for analysis.
Fold of dilution of the sample: 5

-Tissue, Chicken, pork, porcine liver, shrimp or fish (Higher detect:3ppb)
1.Take 3±0.05 g of the homogenized sample into 50 mL centrifuge tube, add 1g NaCI, 6 mL sample Acetonitrile, mix thoroughly with oscillator for 5 min.
2.Take 2 mL supernatant, evaporate to dry at 56℃ in water bath by nitrogen.
3.Add 0.50mL n-Hexane, shake for 30s, add 0.50mL redissolving solution(1×), shake for 30s.
4.Centrifuge at above 4000 r/min at room temperature (20-25 ℃) for 5 min.
5.Remove upper layer, take 50 µL lower layer for analysis.
Fold of dilution of the sample: 0.50

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