1. Principle
Crystal Violet ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of Crystal Violet in tissue, feed and water.
The coupling antigens are coated on the micro-well stripes. The Crystal Violet in the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Crystal Violet antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration.
The optical density (OD) value of the sample has a negative correlation with the Malachite green in it. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the Crystal Violet concentration in the sample.
2. Technical specifications
Sensitivity : 0.05 ppb Detect time: Approx. 75 min
Detection limit:
Tissue ........................................0.10 ppb
Water.........................................0.50ppb
Feed............................................1.0 ppb
Recovery rate:
Tissue and feed......................75 ± 10%
Water.........................................80 ± 20%
Cross-reaction rate:
Crystal Violet..............................................100%
