Principle
DNA Purification Kit is used to purify genomic DNA and total RNA from a single cell and tissue sample. The unique lysate can dissociate cells and inactivate cellular RNA/DNA enzymes to release DNA and RNA complex. RNA is released through filtering genomic DNA-Free spin column and the DNA adsorbs to the Spin column membrane in the presence of high concentrations of chaotropic salt. Genomic DNA is purified from the DNA spin column based on the elution and centrifugation. RNA was selectively adsorbed on the RNA-Free spin column under high disordered salt, is purified through the elution and centrifugation process.
Purified genomic DNA is eluted in low-salt buffer or water, is highly suited for PCR, qPCR, dPCR, NGS, Southern blotting, RAPD, AFLP, and RFLP applications.
Storage conditions and shelf-life
① The kit was dried at room temperature (20-25℃) for 12 months , at 2-8℃ for longer periods.
② RNase Free Dnase I, Proteinase K, DNaseI Buffer, store at -20℃.
Advantage
① Non-polluting: The kit is RNase-Free.
② Particularity: Suitable for animal tissues and cultured cells, can purify RNA and DNA without interfering mutually.
③ Easy operation: All process is simple, the extraction process within 40 minutes.
④ Safety: No need phenol, chloroform and other organic reagents of purification.
⑤ Spin column: Be specific spin column, easy to centrifuge and elute.
⑥ High quality: Effectively guarantee the integrity of RNA/DNA, high efficiency of recycling RNA/DNA, high purity,
no protein and other impurities pollution. It can be used in various molecular biology experiments.
