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Human Papillomavirus Genotyping qPCR Kit

Principle And Intended Use
This product is used for the typing and qualitative detection of 21 subtypes of human papillomavirus (specifically including HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 26, 82, 73, as well as HPV 6, 11, and 81) in cervical exfoliated cell samples from women.

Specifications

12 tests/plate, 5 plates/kit

Description

1  Principle And Intended Use

Based on the quantitative technology platform of multiplex fluorescent PCR, 21 types of HPV are detected using separate tubes for typing and closed tubes for detection. Probes of different colors are labeled for each type of HPV, and only three types are detected in each tube, which can effectively avoid the amplification efficiency differences between types caused by the use of universal primers in traditional typing techniques, as well as the competitive inhibition that may easily occur when all targets are amplified in the same tube.The virus DNA in the sample should be purified and enriched with a nucleic acid extraction and purification reagent before being detected with this kit.


2  Specifications

 12 tests/plate, 5 plates/kit 

 

3  Components

 Each kit contains 1 pre-packaged reagent 96-well plate, 1 microplate sealers,
 1 
HPV Positive Control, 1 HPV Negative Control, and 1 instruction.           

 

4  Storage Condition and Expiring Date

The reagents are stable when stored at room temperature for 12 months without unpacking. Please transport at -25℃ to 35℃. The kit should remain sealed and away from light exposure. Do not use the kit beyond the expiration date.

PCR Assay

① Create a new experiment.

② Select the FAM and VIC channels.

③ Select "none " for Passive Reference (For ABI Real-Time PCR systems).

④ Set cycle conditions. Enter the reaction volume (25μL), and edit the PCR

       program as follows:

       Step 1:50℃ 2min;

       Step 2:95℃ 5min;

       Step 3:95℃ 10s,58℃ 40s (fluorescence collection ).(40 cycles)

⑤ Save the file. Run the program.

        Each well of the reagent strip detects different targets respectively:

Well

Sigal

HPV genotype

A

FAM

HPV16

VIC

HPV18

CY5

HPV31

B

FAM

HPV59

VIC

HPV66

CY5

HPV53

C

FAM

HPV33

VIC

HPV58

CY5

HPV45

D

FAM

HPV56

VIC

HPV52

CY5

HPV35

E

FAM

HPV68

VIC

HPV51

CY5

HPV39

F

FAM

HPV82

VIC

HPV26

CY5

HPV73

G

FAM

HPV6

VIC

HPV11

CY5

HPV81

H

VIC

Internal reference


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