Source | Applications | Cross-reactivity | M.W. ofBcl-xL |
Rabbit | WB, IP, IHC, F | H, M, R, Mk | ~30kD |
Background
Bcl-xL is a protein in Bcl-2 family and plays an important role in apoptosis regulation. It inhibits apoptosis byforming heterodimers with other pro-apoptotic proteins. It can also form oligomeric channels directly on the outer mitochondrial membrane to maintain the normal state of the mitochondrial membrane during cellular stress. Microtubule-damaging agents such as paclitaxel, vinblastine, and nocodazole can inactivate Bcl-xL by phosphorylating it through activation of JNK. The Bcl-x gene can form Bcl-xL and Bcl-xS by alternative splicing. Bcl-xL contains 241 amino acids and has significant homology with Bcl-2, while Bcl-xS has 63 amino acid missing from Bcl-xL in the near transmembrane region. Bcl-xL has an inhibitory effect on apoptosis, and its role in tumorigenesis has been more widely studied.
Cross-Reactivity
Human
Applications
WB | IP | IHC | F |
1:1000 | 1:100 | 1:300 | 1:400 |
Isotype
IgG
Purification
Protein G purified
Immunogen
Synthetic peptide derived from human Bcl-xL.
Storage conditions
Store the Antibody at -20℃ and Primary Antibody Dilution Buffer at 4℃ for up to one year. The Primary Antibody Dilution Buffer can be stored at -20℃for lone-term storage, but freeze-thaw may result in slight turbidity and a small amount of insoluble substance.